Regulatory

Part:BBa_K2765040

Designed by: Fa He   Group: iGEM18_BIT-China   (2018-09-15)

FAFU-CHINA 2020 - Contribution

Seung-pyo Hong et al[1] constructed an expression vector containing FBA1 in Yarrowia Lipolytica (pDMW212 & pDMW214), and measured its GUS Reportor activity and mRNA expression.

  • T--FAFU-CHINA--IMG28.png
    Figure 1. Histochemical and fluorometric GUS reporter assays to measure promoter activity. Fluorometric determination of GUS activity. GUSspecific activity was expressed as nM 4-methylumbelliferone released/min/mg protein.


  • T--FAFU-CHINA--IMG29.png
    Figure 2. Relative quantities of GUS mRNA. The relative transcript levels were calculated using the GUS mRNA level from strains with TEF1::GUS as the reference. Assays were done in quadruplicate.


Reference

[1] Hong S P , Seip J , Walters-Pollak D , et al. Engineering Yarrowia lipolytica to express secretory invertase with strong FBA1IN promoter[J]. Yeast, 2012, 29(2).

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